A simplified genomic subtractive procedure for isolating deleted sequences

نویسندگان

چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A simplified genomic subtractive procedure for isolating deleted sequences.

New approaches have recently been developed for directly identifying genes without knowledge about their function and/or position in a genome (1–3). Among these, representational difference analysis (RDA) (2) is widely used in the detection of deleted DNA in cultured cancer cells (4). However, the procedure is complicated and time-consuming. First, preparation of the tester and the driver invol...

متن کامل

Cloning of deleted sequences (CODE): A genomic subtraction method for enriching and cloning deleted sequences.

The deletion of specific genomic sequences is believed to influence the pathogenesis of certain diseases such as cancer. Identification of these sequences could provide novel therapeutic avenues for the treatment of disease. Here, we describe a simple and robust method called cloning of deleted sequences (CODE), which allows the selective cloning of deleted sequences from complex human genomes....

متن کامل

Identification of Salmonella pullorum genomic sequences using suppression subtractive hybridization.

Pullorum disease affecting poultry is caused by Salmonella enterica serovar Pullorum and results in severe economic loss every year, especially in countries with a developing poultry industry. The pathogenesis of S. Pullorum is not yet well defined, as the specific virulence factors still need to be identified. Thus, to isolate specific DNA fragments belonging to S. Pullorum, this study used su...

متن کامل

Simplified media for isolating Neisseria gonorrhoeae.

Two simplified media for isolating Neisseria gonorrhoeae are described. They differ from the modified Thayer-Martin medium in the enrichment and antibiotics used for preparation. The enrichment for one, enrichment 4 medium, contains only four ingredients, and amphotericin B is used instead of nystatin. This medium is comparable to the modified Thayer-Martin medium for isolating N.gonorrhoeae. I...

متن کامل

A Simplified Procedure for Designing Hermetic Compressors

Research on the various aspects of hermetic compressor design and development has been going on for a long period of time. As old questions get answered, new ones keep coming up. So much work has been done that a beginner in the compressor design field could easily get confused, and might not even know where to start. The purpose of this paper is to present a consolidated and simplified design ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1996

ISSN: 1362-4962

DOI: 10.1093/nar/24.11.2196